In a request for financing genetic examination, the answers to the following questions should be given.
1. Why is genetic examination for this species necessary?
2. Did comparable genetic examination take place already and what were the conclusions (preferably with added references and copies)? For the interpretation of the available examinations it is of course possible to fall back on the expertise within the ESF. Comparable genetic examination is examination on genetic differences in subspecies and subpopulations. It is particularly important to know the exact piece of DNA that was examined (what primer has been used. It is possible that it will be necessary to contact the examiner. Depending on the expertise of the studbook holder, this could be done by the ESF.
3. Are there animals of a known location within the captive population?
4. Are there external morphological aspects known, which could support or replace DNA examination in a later stage?
5. Is it reasonable to expect that still many more founder animals will be added to the studbook? A further comment is that in many cases it is useless to perform DNA examination on an animal bred in captivity if not at least the father can be examined.
6. Are all studbook participants, who are in possession of animals from the wild of this species, prepared to participate in this examination? This includes the contribution and the consequences, which may mean that the animals have to be transferred, for instance when on 1 location no ideal couple can be joined.
7. How many living founder animals are registered in the studbook?
8. How many dead founder animals (females), from which some first generation young still exists, are present in the studbook?
9. How many studbook participants are there and how large is the number of animals to be examined? (In cases where secrecy is demanded, a postal area or town is sufficient).
The questions mentioned above, directed at the applicant are specifically targeted so that the board can answer the following questions.
1. Does the examination yield any value (surplus value) and is there a reasonable expectation of any result?
2. What are the costs of a full examination?
3. Do we have to take into account repeating costs or is it a once only action?
To answer these last questions it is also important to know the viability of the studbook and the relation between the breeding rate and the death rate of the animals.